【英文标准名称】:StandardTestMethodforTotalActiveBiomassinCoolingTowerWaters(KoolKountAssay;KKA)
【原文标准名称】:冷却塔水中总活性生物体的标准试验方法
【标准号】:ASTMD6530-2000(2006)
【标准状态】:现行
【国别】:
【发布日期】:2000
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:colorimetricbioassay;coolingtowerwater;triphenyltetrazoliumchloride;viableactivebiomass
【摘要】:Thistestmethodisusefulforrapiddeterminationofviableactivebiomassconcentrationsincoolingtowerwaters.Theefficiencyofcoolingtowersisdirectlyaffectedbytheconcentrationofbiomassinthecoolingtowerwaters.Asbiomassconcentrationsincrease,biofilmformationoccursresultinginadecreaseintheefficiencyofheatexchangeinthetower.Currenttestsformonitoringthebiomassconcentrationincoolingtowersrequireatleast36hforgrowthofthemicroorganismsonasolidagarsurfaceforcounting.Replicationofmicroorganismsoverthe36-hperiodbeforeresultsareavailablecreatesanaqueousenvironmentwhichisnolongerrepresentedbythedatagenerated.Timelytestresultscanassistinminimizingbiocideadditiontocontrolbiomassconcentrations.KoolKountprovidesdatawithinhourstoallowformoreprecisecontrolofactivebiomassconcentrationsinthewaters.1.1Thistestmethodcoversthedeterminationofviableactivebiomassincoolingtowerwaterintherangefrom102to108cfu/mL(1).Itisasemiquantitativetestmethod.1.2Thistestmethodwasusedsuccessfullywithreagentwater,physiologicsaline,andcoolingtowerwaters.Itistheuser''sresponsibilitytoensurethevalidityofthistestmethodforwatersofuntestedmatrices.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.Forspecifichazardstatements,seeSection.